بررسی اثرات سایتوتوکسیک فراکشن‌های استخراج شده از قارچ صدفی پلئوروتوس فلوریدا بر رده‌های سلولی سرطانی

Received: 3 April, 2009 Accepted: 5 Oct, 2009 Abstract Background & Aim: Nowadays, different medical approaches are used for the treatment of cancers, but in most cases they are not effective or have serious side-effects. This has prompted scientists to look for more effective drugs with less toxicity. This study was to evaluate the cell cytotoxicity effect of fractions isolated from Pleurotus florida on cancer cell lines. Materials & Methods: R5, F5, R10, R30 and R100 fractions were isolated from Pleurotus florida body extract. The growth inhibitory activity of these fractions was determined for different cancer cell lines using colorimetric MTT assay. Results: Results showed that isolated fractions showed significant inhibitory activity for cancer cell lines in a dose-dependent manner. Some of the fractions such as R100 and R30 exhibited more inhibitory activity against HT-29. Among the tested cell lines, HT-29 was very sensitive to these fractions. Conclusion: Isolated fractions particularly R100 fraction from Pleurotus florida body extract showed cytotoxic effect on cancer cell lines. So, they could be studied as anti-cancer agents in tumor-bearing animal models and subsequently in clinical trials for cancer patients. Further studies are needed to elucidate the mechanisms by which R100 fraction act. Keywords: Pleurotus florida, Cancer cell lines, Cytotoxicity Address: Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran Tel: 09354262960 Email: [email protected] Source: Urmia Med J 2010: 21(1): 103 ISSN: 1027-3727